![](https://parts.igem.org/images/partbypart/icon_tag.png)
Tag
Part:BBa_K2760012:Design
Designed by: iGEM18_TecMonterrey_GDL Group: iGEM18_TecMonterrey_GDL (2018-10-09)
6 His tag for E. coli and L. rhamnosus
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Designed both for E. coli and L. rhamnosus, the codon optimization was made with the help of Genscript for both of these organisms.
Source
Comes from optimized sequences of His for E. coli and L. rhamnosus [1]
References
Codon usage in E. coli. (2002) Microbial Genetics Home Page. http://www.sci.sdsu.edu/~smaloy/MicrobialGenetics/topics/in-vitro-genetics/codon-usage.html