Tag

Part:BBa_K2760012:Design

Designed by: iGEM18_TecMonterrey_GDL   Group: iGEM18_TecMonterrey_GDL   (2018-10-09)


6 His tag for E. coli and L. rhamnosus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed both for E. coli and L. rhamnosus, the codon optimization was made with the help of Genscript for both of these organisms.

Source

Comes from optimized sequences of His for E. coli and L. rhamnosus [1]

References

Codon usage in E. coli. (2002) Microbial Genetics Home Page. http://www.sci.sdsu.edu/~smaloy/MicrobialGenetics/topics/in-vitro-genetics/codon-usage.html